ATRX Knockout cell line (143B)

ATRX Knockout cell line (143B) Order

Catalog#

YKO-H1158

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

ATRX Knockout cell line（143B）

Cell Morphology

Epithelial-like, adherent

Passage ratio

1：2-1：3

Species

Human

Gene Name

ATRX

Gene ID

546

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10%FBS

Target region

Knockout region: Exon 9 (based on transcript ATRX-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:143B			STR Info (Cell bank) Cell Line:143B
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	13	13		13	13
D13S317	12	12		12	12
D7S820	11	12		11	12
D16S539	10	13		10	13
VWA	18	18		18	18
TH01	6	6		6	6
AMEL	X	X		X	X
TPOX	11	11		11	11
CSF1PO	12	12		12	12
D12S391	20	20
FGA	24	24
D2S1338	24	25
D21S11	31.2	31.2
D18S51	17	17
D8S1179	11	14
D3S1358	15	15
D6S1043	18	18
PENTAE	7	12
D19S433	13	13
PENTAD	3	9	10.0
D1S1656	13	15

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ATRX gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the 143B cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ATRX gene knockout ATRX Knockout cell line（143B） will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

ATRX Knockout cell line (143B)

Catalog#

YKO-H1158

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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